产品名称：His镍柱,His蛋白纯化介质填料
产品简介：
His镍柱（His蛋白纯化介质填料）产品介绍：
产品英文名称：Ni Sepharose™ 6 Fast Flow - Scale-up Purification
产品标准：5ml体积
产品介绍：
Ni Sepharose™ 6 Fast Flow(GE)具有特异性好、稳定性高、镍离子脱落少、层析介质颗粒粒度均匀、流速快、His标记蛋白结合能量高、使用寿命长、重复性好质量稳定等特点。
His镍柱（His蛋白纯化介质填料） 每ml柱床体积可吸附40mgHis标记蛋白质，可配合注射器或连接液相色谱系统使用。
TECHNICAL SPECIFICATIONS
Matrix Highly crossed-linked 6% agarose, precharged with Ni2+
Average particle size 90 µm
Dynamic binding capacity* Approx. 40 mg histidine-tagged protein/ml medium
Metal ion capacity Approx. 15 µmol Ni2+/ml medium
Compatibility during use Stable in all commonly used buffers, reducing agents, denaturants, and detergents, see Introduction to Tagged Protein Purification for more information.
Chemical stability For one week at 40°C: 0.01 M HCl, 0.1 M NaOH
For 12 h: 1 M NaOH, 70% acetic acid
30 min tested: 30% 2-propanol
1 h tested: 2% SDS
pH stability† 2-14 (short term), 3-12 (long term)
Storage 20% ethanol
Storage temperature 4°C to 30°C
* Protein binding capacity is protein-to protein dependent
† Ni2+-stripped medium
His镍柱（His蛋白纯化介质填料）现货促销。。。。 |