产品名称：荧光素酶

产品简介：

                                                 荧光素酶 荧光素酶

  荧光素酶其实就是一种特殊的蛋白质，通过氨基酸排序的不同，可以反射各种颜色的光。

  荧光素酶（英文名称：Luciferase）是自然界中能够产生生物萤光的酶的统称，其中最有代表性的是一种学名为Photinus pyrali的萤火虫体内的荧光素酶。在相应化学反应中，萤光的产生是来自于荧光素的氧化，有些情况下反应体系中也包括三磷酸腺苷（ATP）

  荧光素酶英文名称：Luciferase firefly；Luciferase from Photinus pyralis (firefly)

  荧光素酶(萤火虫)；重组型酵素

CAS号：61970-00-1

级别：分析纯级AR

product line：BioUltra

Assay：≥98% (SDS-PAGE)

Mol wt：mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)

Protein：10-35% E1%/280

Measuring range：≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)

Foreign activity：ATPase ≤5 nanomolar units/mg protein；Nucleoside diphosphokinase ≤20 nanomolar units/mg protein

荧光素酶活力定义：One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C

产品描述：Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively.

Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units

华越洋生物的荧光素酶性状：粉末。

用途：生化研究。

荧光素酶大量现货促销。。。。。